siTOOLs Biotech Blog

Practical advice in functional genomics

Navigating RNAi off targets: enhancing the precision of gene silencing in therapeutic research.

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This blogpost describes issues encountered in target validation and how to safeguard against poor reproducibility in RNAi experiments.

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Analysis reveals little correlation between Dharmacon siGENOME and ON-TARGETplus, highlighting the complexity of validating RNAi screen hits.

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Exploring siRNA vs shRNA for gene silencing: applications, off-target effects, and why choosing the right RNAi method matters for your research.