Performing target validation well
This blogpost describes issues encountered in target validation and how to safeguard against poor reproducibility in RNAi experiments.
Novel anti-cancer mechanism identified by shRNA/siRNA off-target effects
Unveiling a novel anti-cancer mechanism via siRNA/shRNA off-target effects. Putzbach et al.'s study exposes survival genes in cancer cells, shedding light on CD95/CD95L-induced cell death and the role of miRNA-like activity in off-target effects
CRISPR/Cas9 Screening - The “Copy-Number Effect”
Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.