A Brief Interview with Patrick Hörner on Malaria and insecticide resistance
Malaria, a devastating disease that has plagued humanity for centuries, continues to take a heavy toll on vulnerable populations worldwide.
Similar seed effects in independent siRNA screens
A 2013 study on Parkin translocation used genome-wide siRNA libraries from Ambion (single Silencer Select siRNAs) and Dharmacon (pools of 4 siGENOME siRNAs).
The Iron Law of RNAi Screening
This is the lead singer of a band called Iron Law. He looks like a researcher experiencing massive frustration after discovering what we call the Iron Law of RNAi Screening.
Novel anti-cancer mechanism identified by shRNA/siRNA off-target effects
Unveiling a novel anti-cancer mechanism via siRNA/shRNA off-target effects. Putzbach et al.'s study exposes survival genes in cancer cells, shedding light on CD95/CD95L-induced cell death and the role of miRNA-like activity in off-target effects
CRISPR/Cas9 Screening - The “Copy-Number Effect”
Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.
siRNA vs shRNA - applications and off-targeting
Exploring siRNA vs shRNA for gene silencing: applications, off-target effects, and why choosing the right RNAi method matters for your research.
CRISPR - what can go wrong and how to deal with it
CRISPR's potential pitfalls highlighted: absent phenotypes, inefficient editing, and unexpected results, with strategies for effective management.
