Tag: Off-targets

Addressing off-target effects in genetic research: ensuring precision and accuracy in gene editing technologies.

CRISPR/Cas9 Screening - The “Copy-Number Effect”

Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.