Performing target validation well
This blogpost describes issues encountered in target validation and how to safeguard against poor reproducibility in RNAi experiments.
Little correlation between Dharmacon siGENOME and ON-TARGETplus reagents
Analysis reveals little correlation between Dharmacon siGENOME and ON-TARGETplus, highlighting the complexity of validating RNAi screen hits.
CRISPR/Cas9 Screening - The “Copy-Number Effect”
Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.
