Orthogonal design in software and RNAi screening
Orthogonal design in RNAi screening enhances hit confidence, contrasting with software design principles to minimize systemic interdependencies.
"Phenoville" - RNAi & CRISPR Screening Strategies
Comparing RNAi and CRISPR screening strategies in host-virus interactions. RNAi offers broader insights, CRISPR specificity.
siRNA vs shRNA - applications and off-targeting
Exploring siRNA vs shRNA for gene silencing: applications, off-target effects, and why choosing the right RNAi method matters for your research.
Making sense of siGENOME deconvolution
Deconvolution of Dharmacon siGENOME pools shows surprising results, with low correlation to target genes and significant seed effects.
The final RNAiL?
Explore the debate over RNAi's viability post-CRISPR revelations. Lin et al.'s findings challenge RNAi's efficacy, but nuances suggest RNAi still has value, albeit with precautions against off-target effects
Where's the beef?
Uncover the impact of on- vs. off-target effects in RNAi screening. Analysis of Hasson et al.'s genome-wide screen reveals the prevalence of off-target effects, emphasizing the need for precise siRNA libraries.
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects Classic Papers Series: Lin et al. show RNAi screen dominated...
The nasty, ugly fact of off-target effects
Discover the fascinating history of siRNAs and their impact on gene targeting. Explore the disappointments and lessons learned from siRNA screening.
Don't swallow the fly
There was a PI who screened one s i [RNA], Oh, I don’t know why they screened one s i … siRNA screens have a high false positive rate, due to pervasive off-target effects. Confirming ‘hits’ from single-siRNA screens is a lot of work.
Celebrating 11 years of off-target effects
Celebrating 11 years of off-target effects Celebrating 11 years of off-target effects 2. October 2014 Andrew Walsh This year marks the 11th ...
