CRISPR/Cas9 Screening - The “Copy-Number Effect”
Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.
siRNA vs shRNA - applications and off-targeting
Exploring siRNA vs shRNA for gene silencing: applications, off-target effects, and why choosing the right RNAi method matters for your research.
