Category: CRISPR

5 factors to consider in multi-gene targeting RNAi screens

Effective functional genomic screening depends on a variety of factors that need to be simultaneously addressed to obtain meaningful results. A recent Cell Reports paper demonstrates this by taking a holistic approach to siRNA screening with the use of multi-isoform/multi-gene targeting to address redundant paralogs and pathways in cancer cells.

CRISPR/Cas9 Screening - The “Copy-Number Effect”

Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.

How reproducible are CRISPR screens?

Exploring the reproducibility of CRISPR screens compared to RNAi screens. While CRISPR gRNAs show more consistent gene inhibition, overlap in top hits between CRISPR screens varies, cautioning against overestimating reliability solely based on assay reproducibility.