"Phenoville" - RNAi & CRISPR Screening Strategies
Comparing RNAi and CRISPR screening strategies in host-virus interactions. RNAi offers broader insights, CRISPR specificity.
CRISPR/Cas9 Screening - The “Copy-Number Effect”
Exploring the `copy number effect` in CRISPR/Cas9 screens for essential genes in cancer cell lines. The phenomenon reveals a challenging source of false positives and negatives, prompting the development of computational methods for correction and emphasizing the need for cautious interpretation and complementary validation techniques.
siRNA vs shRNA - applications and off-targeting
Exploring siRNA vs shRNA for gene silencing: applications, off-target effects, and why choosing the right RNAi method matters for your research.
Unexpected Mutations after CRISPR in vivo editing - post-commentary
Delving into concerns surrounding a Nature Commentary on CRISPR-edited mice, including study size, control choice, Cas9 delivery, and variant validation. Critics emphasize the need for rigorous controls and updated databases to assess off-target effects accurately.
Making sense of siGENOME deconvolution
Deconvolution of Dharmacon siGENOME pools shows surprising results, with low correlation to target genes and significant seed effects.
How reproducible are CRISPR screens?
Exploring the reproducibility of CRISPR screens compared to RNAi screens. While CRISPR gRNAs show more consistent gene inhibition, overlap in top hits between CRISPR screens varies, cautioning against overestimating reliability solely based on assay reproducibility.
The final RNAiL?
Explore the debate over RNAi's viability post-CRISPR revelations. Lin et al.'s findings challenge RNAi's efficacy, but nuances suggest RNAi still has value, albeit with precautions against off-target effects
CRISPR - what can go wrong and how to deal with it
CRISPR's potential pitfalls highlighted: absent phenotypes, inefficient editing, and unexpected results, with strategies for effective management.
Where's the beef?
Uncover the impact of on- vs. off-target effects in RNAi screening. Analysis of Hasson et al.'s genome-wide screen reveals the prevalence of off-target effects, emphasizing the need for precise siRNA libraries.
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects Classic Papers Series: Lin et al. show RNAi screen dominated...
