How reproducible are CRISPR screens?
Exploring the reproducibility of CRISPR screens compared to RNAi screens. While CRISPR gRNAs show more consistent gene inhibition, overlap in top hits between CRISPR screens varies, cautioning against overestimating reliability solely based on assay reproducibility.
The final RNAiL?
Explore the debate over RNAi's viability post-CRISPR revelations. Lin et al.'s findings challenge RNAi's efficacy, but nuances suggest RNAi still has value, albeit with precautions against off-target effects
CRISPR - what can go wrong and how to deal with it
CRISPR's potential pitfalls highlighted: absent phenotypes, inefficient editing, and unexpected results, with strategies for effective management.
Where's the beef?
Uncover the impact of on- vs. off-target effects in RNAi screening. Analysis of Hasson et al.'s genome-wide screen reveals the prevalence of off-target effects, emphasizing the need for precise siRNA libraries.
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects
Classic Papers Series: Lin et al. show RNAi screen dominated by seed effects Classic Papers Series: Lin et al. show RNAi screen dominated...
RNA affinity purification: Tips for optimizing
Optimize RNA Affinity Purification: Key strategies for effective isolation, analysis, and insights into RNA interactions.
The limits of chemical modification
Learn how chemical modifications in siRNAs don't always prevent seed-based off-target effects, as evidenced by Rasmussen et al.'s study on Pik3ca siRNA
Deconvoluted SMARTpools are like a box of chocolates
Exploring RNAi screening, Falkenberg et al. uncover the unpredictability of siRNA effects, highlighting the complexity of gene knockdown studies.
Intronic off-target effects with antisense oligos
Exploring ASOs for RNA interference reveals intronic off-target effects, challenging the specificity of antisense oligonucleotide applications.
Simplicity is the ultimate sophistication
siPOOLs embody simplicity in RNAi research, mirroring Apple's philosophy for user-computer harmony, making complex biological insights accessible.
